Exploration of the Lysis Mechanisms of Leukaemic Blasts by Chimaeric T-Cells

Figure 4

z-CD19 chimaeric effectors express mediators of cytotoxicity. (a) CTL and CIK effectors were stained with antigranzyme A, -granzyme B or -perforin antibodies. Transfectant expressions were detected by a goat antihuman IgG (GAH) labeling on CD3⁺ cells. Percentages and MFI are indicated from CD3⁺ cells. (b) CTL and CIK effectors were cocultured in the presence of SupB15 CD19⁺ stimulating cells or Primary B-ALL blasts (AmMax, RoNir and SpSeb). Degranulation of effectors was analyzed by anti-CD107a and -CD107b antibodies gated on CD3⁺ cells. Similarly, IFN-γ and TNF-α specifically produced by responding effectors were analyzed on CD3⁺ gated cells. Similar results were produced with 2 donors of CTL and CIK.

(a)

(b)