Table 1: CTL epitopes identified by using different procedures.
(a) CTL epitopes identified by peptides overlapping by 10 residues and spanning the entire HCV sequence of genotype 1b

 HLA class I allelesRegionAmino acid residuesSequenceHLA restriction

Pt1A*0207,2601 B*3501,4601 CW*0102,0303NS31527–1546WYELTPAETTVRLRAYLNTPB*3501? A*2601?
NS5B2591–2605KMALYDVVSTLPQAVA*0207?
Pt2A*2402,3303 B*4403,5401 CW*0803,1403E1332–351LVVSQLLRIPQAVVDMVAGAB*5401?
NS31638–1656THPITKFVMACMSADLEVVB*5401?
NS5B2591–2605KMALYDVVSTLPQAVn.d.
Pt3A*2602,3101 B*5101,5102 Cw1402,1502NS31373–1380IPFYGKAIB*5101? B*5102?
Pt4A*2402 B*0702,5201 Cw*0702,1202E2611–618YPYRLWHYn.d.
Pt5A*1101,3101 B*6701,5101 Cw*0702,1401NS5A2290–2298RPDYNPPLLB*6701? B*5101?
Pt6A*2402,2601 B*4002 Cw*0304NS2957–964RDWAHAGLB37
NS5A2122–2130FTELDGVRLn.d.
Pt7A*2402,3303 B*0702,3501 Cw*0303,0702Core91–110LGWAGWLLSPRGSRPSWGPTA*3303? B*3501?
Pt8A*2402 B*4801,5201 Cw*0803,1202NS31643–1656KFVMACMSADLEVVn.d.
Pt9A*2402 B*5201 Cw*1202NS41760–1768FWAKHMWNFA*2402
NS5B2556–2564TIMAKNEVFn.d.
NS5B2803–2811LTRDPTTPLn.d.
Pt10A*0201,0301 B*4402,4601 Cw*0102,0501NS41958–1977KRLHQWINEDCSTPCSGSWLn.d.
Pt11A*1101,2601 B*1501,5201 Cw*0401,1202NS41858–1867GVAGALVAFKA*1101?
Pt12A*2402 B*3501,4002 Cw*0303,0304NS31618–1626LHGPTPLLYA*2402?

(b) CTL epitopes identified by HCV-derived synthetic peptides with binding motif of HLA-A24 [51]

 HLA class I allelesRegionAmino acid residuesSequenceHLA restriction

Pt13A*2402,1101 B*3902,5201 Cw*0702,1202NS31375–1385FYGKAIPIEAIn.d.
Pt14A*2402,2601 B*4006,5401 Cw*0801,0803E1284–293VFLVSQLFTFn.d.
E2790–798LYGVWPLLLCw*0801
NS41759–1768AFWAKHMWNFn.d.
NS5A1990–1999DFKTWLQSKLn.d.
NS5A2280–2288KFPPALPIWA*2402
Pt15A*2402,2601 B*3501,4002 Cw*0303,0304NS2910–919PYFVRAQGLICw*0303, 0304
NS2947–956TYVYDHLTPLB*4002
NS31243–1252AYAAQGYKVLCw*0303, 0304
Pt16A*0206,2402 B*5201,5901 Cw*0102,1202NS31443–1451GFTGDFDSVA*0206
Pt17A*2402,3101 B*4801,5101 Cw*0304,0801E2790–798LYGVWPLLLCw*0801
Pt18A*2601,3101 B*3501,5101 Cw*0303,1402NS5B2456–2466VYSTTSRSASLn.d.

(c) CTL epitopes identified by peptides overlapping by 10 residues and spanning the entire HCV sequence [13]

 HLA class I allelesRegionAmino acid residuesSequenceHLA restriction

Pt19A*2602,3101 B*5101,5102 C*1402,1502NS31373–1380IPFYGKAIn.d.
Pt20A*0402 B*0702,5201 C*0702,1202E2611–618YPYRLWHYn.d.
Pt21A*1101,3101 B*6701,5101 C*0702,1402NS5A2290–2298RPDYNPPLLn.d.
Pt22A*2402 B*5201 C*1202NS41759–1768AFWAKHMWNFn.d.
NS5B2556–2564TIMAKNEVFn.d.
NS5B2803–2811LTRDPTTPLn.d.
Pt23A*0201,0301 B*4402,4601 C*0102,0501NS41958–1977KRLHQWINEDCSTPCSGSWLn.d.
Pt24A*2402,4801 B*5201 C*0803,1202NS31637–1656LTHPITKFVMACMSADLEVVn.d.

(d) CTL epitopes identified by peptides overlapping by 10 residues and spanning the entire HCV core sequence

RegionAmino acid residuesSequenceHLA restrictionReference

core88–96NEG(L,M,C)GWAGWB*4403 [49]
core28–36GQIVGGVYLB60 [50]

(e) CTL epitopes identified by HCV-derived synthetic peptides with binding motif of HLA-B*4403

RegionAmino acid residuesSequenceHLA restrictionReference

NS5a2095–2103AEVTQHGSYB*4403 [16]

(f) CTL epitopes identified by comprehensive CTL induction from PBMC of HCV patients

RegionAmino acid residuesSequenceHLA restrictionReference

NS31373–1380IPFYGKAIB*5603 [52]