Aquaporin-6 Expression in the Cochlear Sensory Epithelium Is Downregulated by Salicylates
Figure 3
Effect of aspirin treatment on aquaporin (AQP1, 2, 4, 6, 7) mRNA expression in mouse inner ear. Quantitative real-time RT-PCR of total RNA was performed in mouse inner ear mRNA extract under three different conditions: saline (controls), aspirin (mice treated for 3 days and sacrificed 3 hours after the last injection) recovery (treated for 3 days and sacrificed 24 hours after the last injection). Specific primers for AQP1, 2, 4, 6, 7 were used and qRT-PCR performed as indicated (see Methods). The relative mRNA levels were determined by comparative quantitation (Corbett), the values obtained were normalized to the corresponding -actin level and the results expressed as fold change. Results indicate that mRNA expression level of AQP6 was significantly lower in aspirin treated mice. Bars represent the mean S.E.M. of 4–8 different experiments each from different RNA extracts. *, versus saline and recovery (one-way ANOVA followed by Newman-Keuls’s Q test).
