Autocrine, Not Paracrine, Interferon-Gamma Gene Delivery Enhances Ex Vivo Antigen-Specific Cytotoxic T Lymphocyte Stimulation and Killing

Table 2

Characterization T cell subsets and cytokine expression as percentages, from AAV-infected DC (top panel) and rAAV-infected T cells (bottom panel).

DC treatment	CD8+/CD4	IFN-𝛾/IL4	CD69+, CD8+	CD25+, CD4+
Ctrl (mock)	21.6/51.3	21.85/10.75	27.4	55.6
AAV/CEA	50.3/22.1	26.53/2.73	61.5	16.2
AAV/CEA + exogen IFN-𝛾	52.1/23.6	18.84/2.46	60.8	15.6
AAV/CEA + AAV/ IFN-𝛾	56.5/22.5	27.71/2.90	64.7	14.3
T cell treatment	CD8+/CD4+	IFN-𝛾/IL4	CD69+, CD8+	CD25+, CD4+
AAV/IFN-𝛾	84.6/10.7	43.4/2.37	91.3	17.1
exogen IFN-𝛾	ND	24.28/1.96	ND	ND
Untreated T cells	14.1/69.8	26.53/2.73	20.5	63.5