Three-dimensional Par-C10 acinar-like spheres express TJ as markers of acinar differentiation. Protein expression was detected using immunofluorescence microscopy with goat antimouse occludin (a and d: red), rabbit anti-ZO-1 (b and d: green), followed by Hoechst nuclear stain (c and d: blue). Images were obtained and analyzed using a Carl Zeiss 510 confocal microscope. Sphere diameter taken at the widest point of the plane (I) is shown in m. This model has been published [137].
