A Multifactorial Mechanism in the Superior Antimalarial Activity of -C-GalCer
Figure 6
TCR usage does not affect in vivo V14i NKT cell activation stimulated by -GalCer or -C-GalCer. Groups of 2 WT C57BL/6 were injected i.p. with 1 g of -GalCer or -C-GalCer, or with nothing, and 72 hours later splenocytes and hepatic lymphocytes were isolated and stained for V14i NKT cells using -GalCer-loaded mouse CD1d-IgG1 dimers, as well as for V8.1/8.2, V7, and V2. V14i NKT cells were gated, and FACS analyzed for the three different V gene segments. The numbers shown represent the percentage of cells expressing the indicated V segment. The data shown comes from one of two independent experiments with similar results.