Research Article

Improved Method for In Vitro Secondary Amastigogenesis of Trypanosoma cruzi: Morphometrical and Molecular Analysis of Intermediate Developmental Forms

Figure 2

Morphological analysis of the kinetics of extracellular differentiation from tissue-derived trypomastigotes (a) to amastigotes (h), in hgDMEM at pH 5 without FBS at 37°C: intermediate forms at 1 hour (b), 2 hours (c), 3 hours (d), 4 hours (e), 5 hours (f), and 6 hours (g) of transformation stained with Hema 3. Bar  =  25  𝜇 m.
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