Improved Method for In Vitro Secondary Amastigogenesis of Trypanosoma cruzi: Morphometrical and Molecular Analysis of Intermediate Developmental Forms

Figure 5

Secondary amastigogenesis is accompanied by the gradual acquisition of amastigote-specific Ssp4 surface glycoprotein. The localization of Ssp4 was determined by indirect immunofluorescence in trypomastigotes (a, b), IFs at 1 hour (c, d), 2 hours (e, f), 3 hours (g, h), 4 hours (i, j), 5 hours (k, l) and 6 hours (m, n) of transformation and in amastigotes (o, p). The right panels are the immunofluorescence image (b, d, f, h, j, l, n, p) of the corresponding light microscopy panels (a, c, e, g, i, k, m, o).