Improved Method for In Vitro Secondary Amastigogenesis of Trypanosoma cruzi: Morphometrical and Molecular Analysis of Intermediate Developmental Forms

Table 1

Morphometrical analysis of T. cruzi developmental stages.

Parasite	Parasite length (𝜇m)	Distance between nucleus and kinetoplast (𝜇m)
Trypomastigote	24.17 ±4.06	15.19 ±3.02
If 1 hours	19.04 ±2.62	10.89 ±1.97
If 2 hours	18.52 ±2.43	6.43 ±1.60
If 3 hours	14.84 ±2.47	5.12 ±1.58
If 4 hours	12.52 ±1.03	2.29 ±0.72
If 5 hours	11.98 ±1.44	1.47 ±0.53
If 6 hours	11.26 ±1.24	1.36 ±0.38
Amastigote	11.45 ±1.02	1.08 ±0.22

The results are the average of 100 cells analyzed randomly.