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Journal of Biomedicine and Biotechnology
Volume 2010 (2010), Article ID 314213, 11 pages
http://dx.doi.org/10.1155/2010/314213
Research Article

Electrodelivery of Drugs into Cancer Cells in the Presence of Poloxamer 188

1Institute of Biophysics, Bulgarian Academy of Sciences, Acad. G. Bonchev Street, Building 21, 1113 Sofia, Bulgaria
2German Cancer Research Center, Toxicology and Chemotherapy Unit, Im Neuenheimer Feld 280, 69 120 Heidelberg, Germany
3Centre of Biomedical Engineering “Prof. Ivan Daskalov”, Bulgarian Academy of Sciences, Acad. G. Bonchev Street, Building 103, 1113 Sofia, Bulgaria

Received 13 October 2009; Revised 7 April 2010; Accepted 10 June 2010

Academic Editor: Sonshin Takao

Copyright © 2010 Iana Tsoneva et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

In the present study it is shown that poloxamer 188, added before or immediately after an electrical pulse used for electroporation, decreases the number of dead cells and at the same time does not reduce the number of reversible electropores through which small molecules (cisplatin, bleomycin, or propidium iodide) can pass/diffuse. It was suggested that hydrophobic sections of poloxamer 188 molecules are incorporated into the edges of pores and that their hydrophilic parts act as brushy pore structures. The formation of brushy pores may reduce the expansion of pores and delay the irreversible electropermeability. Tumors were implanted subcutaneously in both flanks of nude mice using HeLa cells, transfected with genes for red fluorescent protein and luciferase. The volume of tumors stopped to grow after electrochemotherapy and the use of poloxamer 188 reduced the edema near the electrode and around the subcutaneously growing tumors.