Electrodelivery of Drugs into Cancer Cells in the Presence of Poloxamer 188

Figure 2

Flow cytometry of MDA-MB-231 cells. Experimental conditions: 5⋅10⁵ cells per 100 𝜇l suspension of 0.3 M mannitol, 0.1 mM Ca acetate and 0.1 mM Mg acetate. Field strength—1.0 kV⋅cm⁻¹, one pulse with a duration of 5 ms. 100 𝜇M propidium iodide (PI) was added during the pulse or after it; (a) nonporated control with PI added; (b) porated control with PI added before the pulse; (c) porated control with PI added after the pulse; (d) and (e) 0.1 mM, (f) and (g) 0.5 mM, (h) and (i) 1.0 mM poloxamer 188 and 100 𝜇M PI added; (d), (f) and (h) are samples electroporated in the presence of poloxamer 188 and PI; (e), (g) and (i) poloxamer 188 and PI were added after the pulse. The M1-marker represents the nonpermeable cells and the M2-marker—the permeable cells.

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