Development of a FLIPR Assay for the Simultaneous Identification of MrgD Agonists and Antagonists from a Single Screen

Figure 3

Assay format for the identification of an agonist and antagonist from a single screen. Test compound is added first followed by a second addition of beta-alanine. An agonist will induce a FLIPR response immediately upon the addition of a test compound. An antagonist will cause a pharmacological blockade of the MrgD receptor resulting in attenuation or complete lack of response to beta-alanine. In the event of no receptor engagement, a normal agonist response will be observed from beta-alanine addition.