Table 5: Gene Ontology analysis of the predicted target genes of 50 miRNAs differentially expressed. Onto-Express analysis on predicted targets of the differentially expressed miRNAs identified enrichment for biological process categories. The gene column indicates the number of predicted targets of the differentially expressed miRNAs upon the number of the targets of all miRNAs considered for the study. Significant differences from the number of targets in each GO category with the expected number of genes were calculated with the assumption of a hypergeometric distribution and P-values were adjusted with the false discovery rate (fdr) correction. 𝑃 < . 0 5 .

RankBiological process categoryGenesCorrected 𝑃 -value

1Cellular process2408/6644.0
 Cell motion148/330.0
 Cell communication908/2223.0
 Cellular component organization546/1383.0
 Cellular developmental process400/949.0
 Cellular metabolic process1563/4269.0
 Regulation of cellular process1555/3840.0
 Cell development183/419.0
 Positive regulation of cellular process371/875.0
 Negative regulation of cellular process394/932.0
 Cell cycle223/5551.0E−5
 Cell death235/5872.0E−5
 Cell proliferation237/6025.0E−5
 Actin-filament based process90/1976.0E−5
 Cell fate commitment45/839.0E−5
 Cell aging14/217.0E−4
 Vescicle-mediated transport158/3977.3E−4
 Cell growth51/112.00286
 Cell fate determination15/23.00286
 Cellular localization224/609.00506
 Gene silencing16/27.00696
 Cell cycle process124/323.00696
 Translational initiation23/48.01253
 Cell fate specification12/20.01728
 Cellular response to stimulus110/292.03290
 Cell adhesion173/479.04094

2Negative regulation of biological process421/992.0
 Negative regulation of metabolic process193/422.0
 Negative regulation to cellular process394/932.0
 Negative regulation of developmental process129/3091.9E−4
 Negative regulation of response to stimulus16/29.01705
 Negative regulation of growth24/53.03564

3Multicellular organismal process820/2037.0
 Multicellular organismal development675/1606.0
 Regulation of multicellular organismal process171/4212.0E−4
 System process227/606.00750
 Respiratory gaseous exchange11/17.01639

4Biological regulation1656/4148.0
 Regulation of molecular function211/478.0
 Regulation of biological process1597/3961.0
 Regulation of biological quality281/7321.3E−4
5Regulation of biological process1597/3961.0
 Regulation of metabolic process850/2038.0
 Regulation of developmental process283/657.0
 Regulation of cellular process1555/3840.0
 Positive regulation of cellular process384/933.0
 Negative regulation of cellular process421/992.0
 Regulation of multicellular organismal process171/4211.4E−4
 Regulation of localization110/2618.7E−4
 Regulation of locomotion41/95.02619
 Regulation of growth64/164.04199

6Metabolic process1631/4509.0
 Biosynthetic process899/2354.0
 Negative regulation of metabolic process193/422.0
 Positive regulation of metabolic process201/473.0
 Regulation of metabolic process1563/2038.0
 Cellular metabolic process1563/4269.0
 Primary metabolic process1551/4187.0
 Macromolecule metabolic process1383/3644.0
 Oxydation reduction52/2555.0E−5
 Catabolic process237/665.01317
 Nitrogen compound metabolic process49/193.03270

7Developmental process821/1967.0
 Multicellular organismal development675/1606.0
 Anatomical structure morphogenesis310/710.0
 Embryonic development140/304.0
 Anatomical structure development584/1379.0
 Cellular developmental process400/949.0
 Regulation of developmental process283/657.0
 Positive regulation of developmental process131/2951.0E−5
 Anatomical structure formation involved in Morphogenesis97/2164.0E−5
 Pattern specification process79/1731.6E−4
 Negative regulation of developmental process129/3091.6E−4
 Pigmentation during development9/13.01264
 Reproductive developmental process31/68.02708
 Aging17/36.04082

8Positive regulation of biological process384/933.0
 Positive regulation of metabolic process201/473.0
 Positive regulation of cellular process371/875.0
 Positive regulation of developmental process131/2951.0E−5
 Positive regulation of homeostatic process6/8.03203

9Localization715/1953.0
 Localization of cell148/330.0
 Macromolecule localization247/6381.1E−4
 Regulation of localization110/2617.7E−4
 Cellular localization224/609.00422
 Establishment of localization577/1657.00463
10Death235/5912.0E−5
 Cell death235/5871.0E−5

11Anatomical structure formation242/6291.1E−4
 Anatomical structure formation involved in  Morphogenesis97/2163.0E−5
 Cellular component assembly165/452.01276

12Response to stimulus464/12762.4E−4
 Response to chemical stimulus185/4655.3E−4
 Response to endogenous stimulus59/136.00633
 Negative regulation to response to stimulus16/29.01844
 Behavior84/215.02638
 Cellular response to stimulus110/292.03534
 Response to stress253/718.03918

13Multi-organism process113/286.00251
 Interspecies interaction between organisms71/172.00565
 Female pregnancy19/39.04504

14Growth96/235.00334
 Cell growth51/112.00298
 Negative regulation of growth24/53.03391
 Regulation of growth64/164.03916

15Locomotion111/277.00422
 Cell motility97/2233.5E−4
 Regulation of locomotion41/95.02439

16Establishment of localization577/1657.00458
 Establishment of protein localization207/5363.3E−4
 Establishment of localization in cell209/576.01045

17Reproduction117/303.00983
 Reproductive process116/301.01127

18Reproductive process116/301.01024
 Reproductive developmental process31/68.03090
 Female pregnancy19/39.04504

19Biological adhesion173/479.03486
 Cell adhesion173/479.03486

20Rhythmic process26/59.04158