Cell Density Plays a Critical Role in Ex Vivo Expansion of T Cells for Adoptive Immunotherapy

Figure 3

ATCs undergo apoptosis at low cell density. (a) Cell viability of ATCs at different cell densities. ATCs were cultured at different cell densities: 1×10⁵, 5×10⁴, and 1×10⁴/mL in 75 cm² flasks for 24 hours. Cell viability was analyzed by flow cytometry. Viable cells are included in the rectangle and percentages of viable cells are indicated within the icon top left. Similar results were obtained in 3 independent experiments. (b) DNA fragmentation of ATCs at different cell densities. The DNA extracts of ATCs cultured at high (1×10⁶/mL) (lane 1) or low (1×10⁴/mL) (lane 2) cell density in 75 cm² flasks for 24 hours were electrophoresed through a 1% agarose gel and stained with ethidium bromide. 100 bp DNA ladder markers were included as markers (M).

(a)

(b)