Research Article

Cell Density Plays a Critical Role in Ex Vivo Expansion of T Cells for Adoptive Immunotherapy

Figure 7

Improved activation with CD28 costimulation at low cell density is accompanied by higher autocrine catalase secretion. Slot blot of culture media from unactivated (lane 1), anti-CD3 antibody (lane 2) and anti-CD3 antibody plus anti-CD28 antibody (lane 3) activated PBMCs at 1 × 1 0 5 /mL at 24 and 48 hours probed with anticatalase antibody.
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