Computational Method for Estimating DNA Copy Numbers in Normal Samples, Cancer Cell Lines, and Solid Tumors Using Array Comparative Genomic Hybridization
Table 5
Comparison of the degree of contamination of cancer cell lines with CEPH cell line NA12776 determined using the picogreen quantitation (averaged over three measurements) and the algorithm presented in this paper.
Cancer cell line
Tissue type
Mix ratio from DNA quantitation
Mix ratio from CN analysis
Percent of SNPs with concordant CN values for contaminated versus pure cancer cell lines
HTB19
Breast
9.6%
97.1%
HTB76
Ovary
18.2%
98.6%
HTB127
Breast
33.6%
98.5%
CCL228*
Colon
43.4%
95.9%
CCL253*
Colon
60.7%
88.2%
HTB119
Lung
54.1%
99.8%
HTB9
Urinary bladder
74.3%
97.0%
HTB41
Salivary gland
84.7%
56.9%
For these two samples one of the picogreen measurements failed.