Figure 5: Quantitative real-time RT-PCR analysis of HO-1 (a), AQP-1 (b), and Bcl-2 (c) expressions in mixed hTECs under basal conditions and after GO-induced oxidative stress (1 IU/mL) either or not in the presence of epoetin delta (100 IU/mL). GAPDH was used as endogenous control housekeeping gene. Preconditioning the cells with EPO resulted in a significant upregulation of HO-1 and AQP-1 mRNA. GO-induced oxidative stress further increased HO-1, AQP-1, and Bcl-2 mRNA expressions with maximum levels 60 minutes after induction of oxidative stress. Data are presented as the mean ± SD of triplicate determinations of 2 runs (i.e., 6 values each). * versus no GO, ° versus epoetin delta 0 IU/mL, # versus 30 minutes GO incubation.