Research Article
Analysis of Adiponectin Gene Polymorphisms in Chinese Population with Systemic Lupus Erythematosus
Table 1
Primer sequences and reaction conditions for genotyping adiponectin polymorphisms.
| Gene | Location | Primer sequence | Product (bp) | Annealing temperature (ºC) | Restriction enzyme | Allele (bp) |
| rs1501299 | Intron-2 | -GTCTAGGCCTTAGTTAATAATGAAGG- | 106 | 56 | STU I | A (106) | -GTGAGAAAGGAGATCCAGGTAA- | C (80,26) | rs2241766 | Exon-2 | -TGGACGGAGTCCTTTGTAGG- | 161 | 56 | Sma I | G (134,27) | -TTGAGTCGTGGTTTCCTGGT- | T(161) |
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