Figure 6: Immunization with a high dose (50 g/mouse) of TcoMSP-D resulting in an increased antigen specific IgG1 antibody response. Groups of 5 BALB/c mice were immunized subcutaneously with TiterMax alone (Control), 5 g, or 50 g of antigen TcoMSP-D (amino-terminal domain) in TiterMax at 4 different sites (25 L/site). Serum was collected at weeks 2, 4, and 6 postimmunization. Sera IgG1 (a) and IgG2a (b) specific for TcoMSP-D were determined by ELISA. The 50 g group showed a significantly higher IgG1 antibody titer than the control group at week 4 and week 6 postimmunization. *; **.