418157.fig.006a
(a)
418157.fig.006b
(b)
Figure 6: Immunization with a high dose (50  𝜇 g/mouse) of TcoMSP-D resulting in an increased antigen specific IgG1 antibody response. Groups of 5 BALB/c mice were immunized subcutaneously with TiterMax alone (Control), 5  𝜇 g, or 50  𝜇 g of antigen TcoMSP-D (amino-terminal domain) in TiterMax at 4 different sites (25  𝜇 L/site). Serum was collected at weeks 2, 4, and 6 postimmunization. Sera IgG1 (a) and IgG2a (b) specific for TcoMSP-D were determined by ELISA. The 50  𝜇 g group showed a significantly higher IgG1 antibody titer than the control group at week 4 and week 6 postimmunization. * 𝑃 . 0 5 ; ** 𝑃 . 0 0 5 .