Research Article

Proteomic Studies of Cholangiocarcinoma and Hepatocellular Carcinoma Cell Secretomes

Table 2

Identified secreted proteins from CCA cell line (HuCCA-1).

BandsAccession numbersNamesProteins were not expressed in HCC cell linesMW/pIScorePeptide matches%CoverageMatched sequencesFunctions

1TSP1_HUMANThrombospondin-1129.30/ 4.717777K.GPDPSSPAFR.ICytoskeleton/mobility
K.FQDLVDAVR.A
K.GGVNDNFQGVLQNVR.F
R.FVFGTTPEDILR.N
R.FTGSQPFGQGVEHATANK.Q
K.GTSQNDPNWVVR.H
R.AQGYSGLSVK.V

2K2C6B_HUMANKeratin, type II cytoskeletal 6B59.96/ 8.0512248R.SGFSSISVSR.SCytoskeleton/mobility
K.FASFIDKVR.F
K.YEELQVTAGR.H
R.ATGGGLSSVGGGSS
TIK.Y

3MYH10_HUMANMyosin-10228.80/ 5.443520K.ESLTK.LCytoskeleton/mobility
K.NDNSSR.F

4LAMC1_HUMANLaminin subunit gamma-1177.49/ 4.824214K.TEQQTADQLLAR.ACytoskeleton/mobility

5MYH10_HUMANMyosin-10228.80/ 5.443730K.NDNSSR.FCytoskeleton/mobility
K.ESLTK.L
R.GCLARK.A
gi 4761370Immunoglobulin lambda light chain variable region10.05/ 9.1736222R.AEDEAYYYCSSYADImmunological response
TKCFGVR.R
K.AFGVR.R
ZNF28_HUMANZinc finger protein 2898.64/ 9.443825K.LEAVGTGIEPKAMSDNA replication/gene regulation
QGLVTFGDVAVDFSQ
EEWEWLNPIQR.N
K.NFQKSSVVIK.Q

6STK10_HUMANSerine/threonine-protein kinase 10112.07/ 6.523611R.ELVAEAKAEVMEEEDGR.DMetabolism
PTPRH_HUMANReceptor-type tyrosine-protein phosphatase H122.28/ 5.213510R.TETRNTTDTR.VMetabolism
Q9HC04_HUMANCytochrome P450 monooxygenase49.50/ 5.983812K.TLDPFETMLK.SMetabolism
MYST4_HUMANHistone acetyltransferase MYST4199.70/ 5.053922K.KPEELLSCADCGSSGHDNA replication/gene regulation
PSCLKFCPELTTNVK.A
R.ANSRQSPAK.V
7PTPRF_HUMANReceptor-type tyrosine-protein phosphatase F213.83/ 5.963721R.YSAPANLYVR.VTransport/binding proteins
R.AAGTEGPFQEVDGV
ATTR.Y
gi 119621568HCG1811249, isoform CRA_a149.08/ 7.765954R.ELTDLNQEFETLQEK.AUnknown
K.EINSLQSDFTK.Y
R.EAELQVDQILTK.S
R.LLIDDQLLR.N
R.LSLSPEVLDLTSNSLK.R
ITA2_HUMANIntegrin alpha-2129.22/ 5.103832R.FGIAVLGYLNR.NTransport/binding proteins
K.IPLLYDAEIHLTR.S
K.IGQTSSSVSFK.S

8LAMB3_HUMANLaminin-5 beta 3129.33/ 7.303621K.IQEVGEITNLR.VTransport/binding proteins
R.LPNVDLVLSQTK.Q

9ITA6_HUMANIntegrin alpha-6118.82/ 6.363611K.DEITFVSGAPR.ATransport/binding proteins

10RAP2B_HUMANRas-related protein Rap-2b20.49/ 4.5240114K.EIEVDSSPSVLEILDTAGTEQFASMR.DSignal transduction
RAP2C_HUMANRas-related protein Rap-2c20.73/ 4.6740114Signal transduction
RAP2A_HUMANRas-related protein Rap-2a20.60/ 4.5240114Signal transduction

11EF2_HUMANElongation factor 295.28/ 6.393633R.VFSGLVSTGLK.VProtein synthesis and degradation
M.VNFTVDQIR.A
K.YEWDVAEAR.K
ACTN4_HUMANAlpha-actinin-4102.20/ 5.278779R.DLLLDPAWEK.QTransport/binding proteins
K.LASDLLEWIR.R
K.ASIHEAWTDGK.E
R.EAILAIHK.E
R.QFASQANVVGPWIQ
TK.M
K.GISQEQMQEFR.A
R.ETTDTDTADQVIASFK.V

12DSG4_HUMANDesmoglein-4113.75/ 4.424312R.EGIKGSSLLNYVLGTYTransport/binding proteins
TAIDLDTGNPATDVR.Y
13gi 153792590Heat shock 90 kDa protein 198.10/ 5.0797610R.ELISNSSDALDK.IChaperone/stress response
K.EDQTEYLEER.R
K.YIDQEELNK.T
K.TKPIWTR.N
K.HIYYITGETK.D
K.DQVANSAFVER.L

14EZRI_HUMANEzrin69.37/ 5.944246K.APDFVFYAPR.LCytoskeleton/mobility
K.IALLEEAR.R
R.QLLTLSSELSQAR.D
K.IGFPWSEIR.N
gi 386758GRP78 precursor72.29/ 5.076147R.ITPSYVAFTPEGER.LChaperone/stress response
K.TFAPEEISAMVLTK.M
R.IINEPTAAAIAYGLDK.R
R.LTPEEIER.M

15TRFL_HUMANLactotransferrin78.13/ 8.013512R.YYGYTGAFR.CTransport/binding proteins
MOES_HUMANMoesin67.78/ 6.084323R.EDAVLEYLK.ICytoskeleton/mobility
K.APDFVFYAPR.L

16Q53GZ6_HUMANHeat shock 70 kDa protein 8 isoform 1 variant70.85/ 5.379658R.TTPSYVAFTDTER.LChaperone/stress response
K.SFYPEEVSSMVLTK.M
K.DAGTIAGLNVLR.I
R.FEELNADLFR.G
K.LLQDFFNGK.E

17ALBU_HUMANHuman serum albumin69.32/ 5.866133K.VPQVSTPTLVEVSR.NTransport/binding proteins
R.LHCASLQK.F
K.KVPQVSTPTLVEVSR.N

18Q16503_HUMANThyrotropin receptor-I28.44/ 6.952512K.ELPLLK.FSignal transduction
19KPYM_HUMANPyruvate kinase isozymes M1/M257.88/ 7.95119713K.GSGTAEVELKK.GMetabolism
K.ITLDNAYMEK.C
K.IYVDDGLISLQVK.Q
K.GVNLPGAAVDLPA
VSEK.D
K.DIQDLK.F
R.GDLGIEIPAEK.V
K.VFLAQK.M

20PDIA3_HUMANProtein disulfide-isomerase A356.75/ 5.923612R.ELSDFISYLQR.EMetabolism
PDIA1_HUMANProtein disulfide-isomerase56.64/ 6.104026K.TFSHELSDFGLESTAProtein synthesis and degradation
GEIPVVAIR.T
R.FLQDYFDGNLK.R

21HERC6_HUMANProbable E3 ubiquitin-protein ligase HERC646.39/ 7.193912K.LTLDEK.KMetabolism
RBM27_HUMANRNA-binding protein 2744.78/ 9.544113K.TSSAVSTPSKVK.TDNA replication/gene regulation
K2C7_HUMANKeratin, type II cytoskeletal 751.39/ 5.424525R.EVTINQSLLAPLR.LCytoskeleton/mobility
R.LPDIFEAQIAGLR.G
CATD_HUMANCathepsin D44.52/ 6.0977616R.TMSEVGGSVEDLIAK.GProtein synthesis and degradation
K.QPGITFIAAK.F
K.FDGILGMAYPR.I
K.LVDQNIFSFYLSR.D
K.VSTLPAITLK.L
K.LSPEDYTLK.V

22CATD_HUMANCathepsin D44.52/ 6.098849K.LVDQNIFSFYLSR.DProtein synthesis and degradation
K.QPGITFIAAK.F
R.YYTVFDR.D
R.VGFAEAAR.L
EF1A1_HUMANElongation factor 1-alpha 147.43/ 9.004925R.EHALLAYTLGVK.QProtein synthesis and degradation
K.IGGIGTVPVGR.V
TBB5_HUMANTubulin beta chain49.73/ 4.754324R.ISVYYNEATGGK.YCytoskeleton/mobility
R.FPGQLNADLR.K
23ENOA_HUMANAlpha-enolase47.14/ 7.17100615R.AAVPSGASTGIYEAMetabolism
LELR.D
K.KLNVTEQEK.I
K.LMIEMDGTENK.S
K.GVPLYR.H
K.LAMQEFMILPVGAA
NFR.E
K.YDLDFK.S
gi 119590453EDAR-associated death domain, isoform CRA_a42.32/ 5.644225K.KLNVTEQEK.IUnknown
K.LMIEMDGTENK.S

24ACTB_HUMANActin, cytoplasmic 141.71/ 5.1410739K.AGFAGDDAPR.ACytoskeleton/mobility
R.GYSFTTTAER.E
K.SYELPDGQVITIGNER.F
K1C18_HUMANKeratin, type I cytoskeletal 1848.03/ 5.164013R.AQIFANTVDNAR.ICytoskeleton/mobility

25ACTS_HUMANActin, alpha skeletal muscle42.02/ 5.23123714K.AGFAGDDAPR.ACytoskeleton/mobility
R.AVFPSIVGRPR.H
K.RGILTLK.Y
R.GILTLK.Y
K.IWHHTFYNELR.V
R.LDLAGR.D
K.EITALAPSTMK.I
ACTB_HUMANActin, cytoplasmic 141.71/ 5.29162722K.AGFAGDDAPR.ACytoskeleton/mobility
R.AVFPSIVGRPR.H
K.RGILTLK.Y
K.IWHHTFYNELR.V
R.VAPEEHPVLLTEAP
LNPK.A
R.LDLAGR.D
R.GYSFTTTAER.E
K.EITALAPSTMK.I

26SH2D7_HUMANSH2 domain-containing protein 749.78/ 5.963511R.MNQAR.LTransport/binding proteins
K2C8_HUMANKeratin, type II cytoskeletal 830.84/ 4.913714R.LEGLTDEINFLR.QCytoskeleton/mobility

27DJB11_HUMANDnaJ homolog subfamily B member 1140.49/ 5.743613R.TLEVEIEPGVR.DChaperone/stress response
28gi 10567590Sodium bicarbonate cotransporter-like protein118.58/ 8.004524R.LCHAQSRSMNDISLTTransport/binding proteins
PNTDQR.K
R.LLGNSCKFIPDLALM
SFILFFGTYSMTLTLK.K
ALDOA_HUMANFructose-bisphosphate aldolase A39.40/ 8.3052520M.PYQYPALTPEQK.KMetabolism
K.ELSDIAHR.I
K.GILAADESTGSIAK.R
R.QLLLTADDR.V
R.TVPPAVTGITFLSGG
QSEEEASINLNAINK.C

29AFF4_HUMANAF4/FMR2 family member 469.38/ 9.453811K.NSSSTSKQK.KDNA replication/gene regulation
ANXA2_HUMANAnnexin A238.55/ 7.5772724R.DALNIETAIK.TSignal transduction
K.GVDEVTIVNILTNR.S
R.QDIAFAYQR.R
K.SALSGHLETVILGLLK.T
K.TPAQYDASELK.A
R.TNQELQEINR.V
K.DIISDTSGDFRK.L
ANXA1_HUMANAnnexin A138.69/ 6.633613K.TPAQFDADELR.ASignal transduction
gi 182073Erythroid protein 4.1 isoform A86.55/ 5.314222K.APIAAPEPELK.TMetabolism
K.TVKGGISETR.I

30ANXA2_HUMANAnnexin A238.55/7.5770721K.AYTNFDAER.DSignal transduction
R.DALNIETAIK.T
K.GVDEVTIVNILTNR.S
R.QDIAFAYQR.R
K.TPAQYDASELK.A
R.TNQELQEINR.V
K.SLYYYIQQDTK.G
ANXA1_HUMANAnnexin A138.69/ 6.6352415K.GVDEATIIDILTK.RSignal transduction
K.TPAQFDADELR.A
R.SEDFGVNEDLADSDAR.A
K.GTDVNVFNTILTTR.S
G3P_HUMANGlyceraldehyde-3-phosphate dehydrogenase36.03/3626K.VGVNGFGR.IMetabolism
8.26R.GALQNIIPASTGAAK.A
K1C9_HUMANKeratin, type I cytoskeletal 962.09/ 5.193524R.SGGGGGGGLGSGGSIR.SCytoskeleton/mobility
K.STMQELNSR.L
31ANXA1_HUMANAnnexin A138.69/ 6.6347314K.GVDEATIIDILTK.RSignal transduction
K.GGPGSAVSPYPTFNP
SSDVAALHK.A
K.TPAQFDADELR.A
ANXA5_HUMANAnnexin A535.91/ 4.733514R.GTVTDFPGFDER.ASignal transduction

32HRX_HUMANHistone-lysine N-methyltransferase HRX431.68/ 9.233630R.GRPPSTERIK.TDNA replication/gene regulation
R.EPTFR.W
R.GPRIK.H

33A8K1N0_HUMANTyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide, isoform CRA_a27.73/ 4.7377522K.SVTEQGAELSNEER.NMetabolism
R.NLLSVAYK.N
K.VFYLK.M
K.GIVDQSQQAYQ
EAFEISK.K
K.DSTLIMQLLR.D

34gi 119610955TSR1 20S rRNA accumulation homolog yeast isoform CRA b50.28/ 9.113812K.LLLLDTQQEAGMLLR.QDNA replication/gene regulation
K1C18_HUMANKeratin, type I cytoskeletal 1847.31/ 5.274024R.AQIFANTVDNAR.ICytoskeleton/mobility
R.IVLQIDNAR.L

35TPIS_HUMANTriosephosphate isomerase26.65/ 6.5047211R.HVFGESDELIGQK.VMetabolism
K.VVLAYEPVWAIGTGK.T

36FLNA_HUMANFilamin-A isoform CRA_e263.75/ 5.793520K.DGTVTVR.YCytoskeleton/mobility
K.RDSLPR.L
Z804A_HUMANZinc finger protein 804A136.80/ 8.194922M.ECYYIVISSTHLSNGDNA replication/gene regulation
HFRNIK.G
R.SLVLQNDMK.H
A0N4V7_HUMANHCG20397972.21/ 9.7936138K.GITLSVRP.-Unknown
gi 119569964HCG204048721.65/ 8.6039110R.IQGKDLTVWTTQDVUnknown
EGILGAK.G

37PRDX1_HUMANPeroxiredoxin-122.10/ 8.2745315R.TIAQDYGVLK.AProtection and detoxification
R.QITVNDLPVGR.S
R.LVQAFQFTDK.H
gi 16075531Immunoglobulin kappa chain variable region9.11/ 9.1336113R.SGTDFTLKISR.VImmunological response
38NOV_HUMANNephroblastoma overexpressed precursor39.14/ 8.123927K.QTRLCMVRPCEQEPDNA replication/gene regulation
EQPTDK.K
K.AIHLQFK.N
NGAL_HUMANNeutrophil gelatinase-associated lipocalin, NGAL, Lipocalin 220.54/ 8.873816K.SYPGLTSYLVR.VTransport/binding proteins
PRDX1_HUMANPeroxiredoxin-122.10/ 8.2739315R.TIAQDYGVLK.AProtection and detoxification
R.QITVNDLPVGR.S
R.LVQAFQFTDK.H

39DESP_HUMANDesmoplakin201.24/ 6.684021K.EISMQKEDDSK.NTransport/binding proteins
R.GIVDSITGQR.L
NGAL_HUMANNeutrophil gelatinase-associated lipocalin, NGAL, Lipocalin 220.54/ 8.8742211K.MYATIYELK.ETransport/binding proteins
K.SYPGLTSYLVR.V

40Q59FK3_HUMANAnnexin IV variant25.41/ 6.034126-.AASISR.LSignal transduction
K.GDTSGDYR.K
FAM3C_HUMANProtein FAM3C24.67/ 8.523717R.LIADLGSTSITNLGFR.DSignal transduction

41K1C16_HUMANKeratin, type I cytoskeletal 1651.24/ 4.7974615R.APSTYGGGLSVSSR.FCytoskeleton/mobility
R.LASYLDK.V
R.ALEEANADLEVK.I
K.IIAATIENAQPILQID
NAR.L
K.ASLENSLEETK.G
R.LEQEIATYR.R
K1C9_HUMANKeratin, type I cytoskeletal 962.09/ 5.196858R.SGGGGGGGLGSGGSIR.SCytoskeleton/mobility
R.LASYLDK.V
K.TLLDIDNTR.M
R.QGVDADINGLR.Q
R.QEYEQLIAK.N

42A8KAH9_HUMANRas-related protein Rap-1A20.97/ 6.3842214K.SALTVQFVQGIFVEK.YSignal transduction
K.INVNEIFYDLVR.Q
43gi 150023192NADH dehydrogenase subunit 567.02/ 9.044111R.KMVGLLK.TMetabolism
gi 62906706Diacylglycerol kinase alpha28.08/ 4.983512K.KVSDVLK.LMetabolism
LIPH_HUMANLipase member H50.83/ 7.143511R.KVAMVLK.EMetabolism
gi 119586924Rotatin isoform CRA_e44.01/ 9.193611K.KEDGVIK.ETransport/binding proteins

44gi158430561Chain A, Crystal Structure Of The Bard1 Brct Repeat24.10/ 8.623713K.YEIPEGPR.RDNA replication/gene regulation

45PRDX5_HUMANPeroxiredoxin-5, mitochondrial22.01/ 8.753815R.LLADPTGAFGK.EProtection and detoxification
Q05D03_HUMANRHOV protein27.79/ 8.934027R.AMPPR.ETransport/binding proteins
R.ACCYLECSALTQK.N

46PROF1_HUMANProfilin-115.04/ 8.4578441K.TFVNITPAEVGVLVGK.DCytoskeleton/mobility
R.SSFYVNGLTLGGQK.C
R.DSLLQDGEFSMDLR.T
K.STGGAPTFNVTVTK.T

47HBB_HUMANHemoglobin subunit beta15.99/ 6.8739218R.LLVVYPWTKR.FTransport/binding proteins
K.LHVDPENFR.-

48gi 119619136HCG1790904, isoform CRA_b37.27/ 9.674024K.SSILYRHK.VUnknown
K.NEMLTFK.A
CENPF_HUMANCentromere protein F22.41/ 7.113510K.YTALEQKLK.KCell cycle