439174.fig.004a
(a)
439174.fig.004b
(b)
Figure 4: Fluorescent staining of L. intermedia (a) and L. whitmani (b) midguts incubated with PGs from procyclic (PRO) and metacyclic (META) L. braziliensis, probed with CA7AE antibody (1:400) and developed with FITC (1:1000). Control midguts were incubated with primary and secondary antibodies only.