Differential Proteome Analysis of the Preeclamptic Placenta Using Optimized Protein Extraction
Figure 3
Two-dimensional separation of precipitated proteins (100 g) in PE placenta solubilized in urea/CHAPS. Segments show differential expression pattern in consequence of precipitation procedures. (a) dichloromethane/methanol, (b) TCA, (c) TCA followed by ethanol wash, (d) acetone, (e) acidified acetone, and (f) ethanol. Circles indicate landmarks for orientation. The gels were stained with silver.