Cytokines and Growth Factors Stimulate Hyaluronan Production: Role of Hyaluronan in Epithelial to Mesenchymal-Like Transition in Non-Small Cell Lung Cancer
Figure 5
Exogenous HA and overexpression of HAS3 gene reduces sensitivity to EGFR-TKI in A549 and H358 cell lines. (a) A549 cells were seeded in a 96-well flat bottom plates and treated with either 250βg/mL or 500βg/mL of 132βkDa HA in the absence or presence of varying concentration of EGFR-TKI for 72 hours. To determine whether this reduced sensitivity is due to HA, 10βunit/mL of Streptomyces hyaluronidase were coincubated with 500βg/mL HA and treated with varying concentration of EGFR-TKI for 72 hours. The in vitro EGFR-TKI sensitivity of A549 cells was evaluated by cell proliferation assay. (b) HAS3 and vector-only control were cultured for 48 hours before treatment in the absence or presence of varying concentration of EGFR-TKI for 72 hours before assaying for cell growth. The experiments were repeated three times. A representative is shown for (a) and (b). (Values are , , .)
