High Prevalence of Arcobacter Carriage in Older Subjects with Type 2 Diabetes
Figure 1
Example of the PCR analysis of DNA extracted from the 3 reference strains and of five representative faecal samples using the species specific primers. Lane M, 100-bp ladder; lanes 1–3 PCR products of the control strains: A. butzleri ATCC 4961 (401-bp), A. cryaerophilus ATCC 43157 (257-bp), and A. skirrowii ATCC 5113 (641-bp), respectively. Lanes A, F, N, and B, G, O results obtained for samples 1 and 2, respectively, showing only amplicons of the expected size for A. cryaerophilus; lanes C, H, P and D, I, Q, results obtained from samples 3 and 4, respectively, showing only amplicons of the expected size for A. butzleri; lanes E, L, R, results obtained from sample 5 showing amplicons of the expected size for A. butzleri and A. cryaerophilus. None of the samples showed amplicons with the A. skirrowii-specific primers; lanes 4 to 6, negative controls.
