497219.fig.002
Figure 2: Schematic diagram of immunization study design. Six groups of mice (groups of 6) were immunized with different vaccine preparations as indicated. Group 1: a mixture of 50  𝜇 g DNA vector pCAGGS and 50  𝜇 g SIV Gag-VLPs; Group 2: 50  𝜇 g HIV Env-DNA; Group 3: 50  𝜇 g SHIV 89.6 VLPs; Group 4: a mixture of 50  𝜇 g HIV Env-DNA and 50  𝜇 g SHIV 89.6 VLPs; Group 5: simultaneous injection with 50  𝜇 g HIV Env-DNA and 50  𝜇 g SHIV 89.6 VLPs at separate sites; Group 6: a mixture of 50  𝜇 g pCAGGS DNA vector and 50  𝜇 g SHIV 89.6 VLPs; Group 7: a mixture of 50  𝜇 g HIV Env-DNA and 50  𝜇 g SIV Gag-VLPs. Mice were immunized twice by intramuscular injections at weeks 0 and 4, and sacrificed at week 6 (two weeks after the second immunization) to collect blood samples and prepare splenocytes for analysis of immune responses.