The Unexpected Role for the Aryl Hydrocarbon Receptor on Susceptibility to Experimental Toxoplasmosis

Figure 4

STAg-induced proliferative responses of spleen cells. Isolated spleen cells from T. gondii-infected (25 days p.i.) WT or AhR-/- mice were stimulated with 2.5 𝜇g/mL of STAg for 5 days in vitro. ³[H]TdrU was added (0.5 𝜇Ci/well) 20 hours before harvesting, and counts per minute (cpm) were calculated using a liquid scintillation counter (a). Secretion of IL-2 (b), IL-12 (c), and IFN-γ (d) in supernatants recovered from cell cultures were evaluated by an ELISA-sandwich. Means + SE, 𝑛=5.^∗𝑃<.05, Student’s t-test.

(a)

(b)

(c)

(d)