Reduced Expression of TCR Zeta Is Involved in the Abnormal Production of Cytokines by Peripheral T Cells of Patients with Systemic Lupus Erythematosus
Figure 2
RT-PCR analysis of gene expression of IFN- upon stimulation with an anti-CD3 antibody. Control T cells (Control) and SLE T cells (SLE) were cultured in the presence (+CD3) or absence (None) of stimulation. Twenty ng of total RNAs extracted from the cells were subjected to RT-PCR for IFN- and GAPDH. The resultant DNA fragments were separated through a 1.5% agarose gel and stained with cyber green.