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Journal of Biomedicine and Biotechnology
Volume 2010 (2010), Article ID 510978, 5 pages
http://dx.doi.org/10.1155/2010/510978
Research Article

Budding of Taenia crassiceps Cysticerci In Vitro Is Promoted by Crowding in Addition to Hormonal, Stress, and Energy-Related Signals

Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, A.P. 70228, C.P. 04510, México D.F., Mexico

Received 19 August 2009; Accepted 23 October 2009

Academic Editor: Jorge Morales-Montor

Copyright © 2010 Pedro Ostoa-Saloma et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Taenia crassiceps cysticerci (cysts) reproduce by budding. The cysts' production of buds was measured in vitro to explore parasite and environmental-related factors involved in the extreme individual variation in parasite loads of inbred mice. Cysts were placed in in vitro culture for 10 days at initial parasite densities of 1, 5, 10 cysts/well in 1 ml of RPMI Medium 1640 without serum. Results showed that there is considerable intrinsic initial variation among inoculated cysts in their production of buds and that increasing parasite density (crowding) stimulates the overall production of buds and recruit into budding most of the cysts. Identical cultures were then subjected to various treatments such as heating and exposure to peroxide to induce stress, or to 17ß-estradiol, insulin, glucose, or insulin+glucose to supplement putatively limiting hormonal and energy resources. All treatments increased budding but the parasites' strong budding response to crowding alone overshadows the other treatments.