Decrease of Klotho in the Kidney of Streptozotocin-Induced Diabetic Rats

<table>Alteration of superoxide generation (ROS) in the kidneys of STZ-diabetic rats treatmented with tiron. Klotho protein in the kidneys of STZ-diabetic rats treatmented with isulin, phlorizin and tiron. (a) Lucigenin assay of the STZ-diabetic rats. Western blot analysis of (b) Klotho expression in the kidneys of STZ-diabetic rats. Upper picture shows protein levels or the corresponding <svg height="13.425" id="M63" style="vertical-align:-2.29482pt" version="1.1" viewbox="0 0 8.8500004 13.425" width="8.8500004" xmlns="http://www.w3.org/2000/svg">
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</svg>-actin levels shown as loading controls in kidneys isolated from STZ-diabetic rats. The different treatments are described in materials and methods. Quantification of protein level using Klotho/<i>β</i>-actin, expressed as means with SE (<svg height="10.8125" id="M64" style="vertical-align:-0.10033pt" version="1.1" viewbox="0 0 35.099998 10.8125" width="35.099998" xmlns="http://www.w3.org/2000/svg">
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</svg> per group) in each column are presented in the lower panel. <svg height="13.7375" id="M65" style="vertical-align:-1.76814pt" version="1.1" viewbox="0 0 139.7 13.7375" width="139.7" xmlns="http://www.w3.org/2000/svg">
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<tspan style="font-size: 12.50px; " x="27.106504" y="0">.</tspan>
<tspan style="font-size: 12.50px; " x="30.232254" y="0">0</tspan>
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</svg> compared to the value of vehicle-treated normal rats.</table>

BioMed Research International

fig2

Figure 2

Figure 2: Decrease of Klotho in the Kidney of Streptozotocin-Induced Diabetic Rats 