Figure 5: Changes in Klotho expression levels in cultured MDCK cells. (a) Western blot analysis of Klotho expression in normal glucose (5 mmol/L) medium in MDCK cells treated with . Upper picture shows the protein level of Klotho or actin in MDCK cells. compared to MDCK cells without treatment. Western blot analysis was used to detect Klotho (b) expression in MDCK cells cultured with normal glucose (5 mmol/L; control) or high glucose (40 mmol/L glucose; HG) medium with or without tiron (10 mmol/L) treatment. Upper picture shows the protein level of Klotho or actin in MDCK cells. Quantification of protein levels indicated as means with SE ( per group) in each column are presented in the lower panel. compared to control.