513853.fig.005a
(a)
513853.fig.005b
(b)
Figure 5: Changes in Klotho expression levels in cultured MDCK cells. (a) Western blot analysis of Klotho expression in normal glucose (5 mmol/L) medium in MDCK cells treated with H 2 O 2 . Upper picture shows the protein level of Klotho or actin in MDCK cells. 𝑃 < . 0 0 1 compared to MDCK cells without H 2 O 2 treatment. Western blot analysis was used to detect Klotho (b) expression in MDCK cells cultured with normal glucose (5 mmol/L; control) or high glucose (40 mmol/L glucose; HG) medium with or without tiron (10 mmol/L) treatment. Upper picture shows the protein level of Klotho or actin in MDCK cells. Quantification of protein levels indicated as means with SE ( 𝑛 = 4 per group) in each column are presented in the lower panel. 𝑃 < . 0 0 1 compared to control.