The Vacuolar-Type H⁺-ATPase in Ovine Rumen Epithelium is Regulated by Metabolic Signals

Figure 7

Immunoblot of the vH⁺-ATPase subunit B (≈60 kDa) and the Na⁺/K⁺-ATPase 𝛼 subunit (≈110 kDa) in ovine REC. Protein extracts were prepared from REC isolated during series 1 (lane 1 and 4) or series 2 (lane 2, 3, 5 and 6) of the experiments. For the Na⁺/K⁺-ATPase 𝛼 subunit, a band at the expected size of approximately 110 kDa was always detected (lane 4–6). In contrast, a clear 60 kDa-band for H⁺-ATPase subunit B protein was found with protein extracts from series 1 only (lane 1). With protein extracts from REC with low metabolic activity, staining was very weak (lane 2) or completely absent (lane 3).