Ulvan, a Sulfated Polysaccharide from Green Algae, Activates Plant Immunity through the Jasmonic Acid Signaling Pathway

Table 3

Defense genes upregulated after ulvan or MeJA treatments of M. truncatula.

ID-Mt16kPlus	TIGR Gene Index v8	Annotation	Ratio		P-value
			Ulvan	MeJA	Ulvan	MeJA
MT004351	TC104560	Phospholipase D	1.72	1.66	0.036	0.046
MT014135	BF640256	Lipoxygenase	1.51	1.50	0.011	0.011
MT015543	TC107737	Wound-induced protein	1.54	1.57	0.038	0.031
MT006789	TC104515	Defensin	2.31	1.85	0.001	0.006
MT008333	TC95553	HRGP-like	2.08	2.01	0.032	0.039
MT007566	TC100849	Pectinesterase inhibitor	1.80	1.51	0.003	0.022
MT000246	TC94445	Trypsin protease inhibitor	37.31	6.41	0.010	0.018
MT000087	TC106371	Protease inhibitor	1.83	1.73	0.026	0.020
MT005382	TC111698	Phytochelatin synthetase-like protein	1.55	1.75	0.015	0.003
MT014728	TC131819	Peroxidase	1.68	1.67	0.030	0.025
MT002340	TC96537	Thioredoxin reductase	1.75	1.74	0.035	0.038
MT004461	TC95580	Heat shock protein	1.67	2.16	0.015	0.001
MT015962	TC105992	Lipase GDSL	1.51	1.52	0.037	0.034
MT000987	TC101355	Anthranilate N-benzoyltransferase-like	1.51	1.52	0.013	0.011
MT014991	TC99521	Tropinone reductase	1.53	1.77	0.014	0.002
MT006133	TC98074	4-coumarate-CoA ligase	1.71	1.85	0.001	0.000

M. truncatula plants were sprayed with ulvan (ethanol-insoluble fraction at 1 mg⋅mL^-1) or MeJA (500 𝜇M) with Actilandes at 0.1%. Leaves collected 1 and 2 days after treatment were pooled and other samples were collected after 6 days. The fold ratio is calculated with the corresponding Actilandes-treated sample.