Nonintegrating Lentiviral Vector-Based Vaccine Efficiently Induces Functional and Persistent CD8+ T Cell Responses in Mice

Figure 1

HIV-based transfer vectors plasmid used in this study. The internal CMV promoter drives the expression of the codon-optimized HIV_JR-FL gp120 Envelope protein and the murine GM-CSF (mGM-CSF). The packaging signal (Ψ), primer binding site (PBS), major splice donor (SD) and acceptor (SA) sites, bovine Growth Hormone polyadenylation signal (bHGpA), central polypurine tract (cPPT), internal ribosomal entry site from poliovirus (pIRES), and Woodchuck posttranscriptional regulatory element (WPRE) are indicated.