Nonintegrating Lentiviral Vector-Based Vaccine Efficiently Induces Functional and Persistent CD8+ T Cell Responses in Mice

Figure 2

T cell responses measured on splenocytes (a) and BM-derived cells (b) at 90 days from immunizations. Results from one representative experiment out of three independent experiments are shown. IFN-𝛾 ELISPOT was performed on cells stimulated overnight with either H-2^d restricted specific Envelope JR-9mer peptide or H-2^d restricted unrelated GFP-9mer peptide. IFN-𝛾 producing T cells are expressed as SFC/10⁶ cells after background subtraction (see Section 2). The error bars indicate the standard deviation among four mice of the same group, and 𝑃-values are indicated for intergroup comparisons.

(a)

(b)