Figure 2: T cell responses measured on splenocytes (a) and BM-derived cells (b) at 90 days from immunizations. Results from one representative experiment out of three independent experiments are shown. IFN- ELISPOT was performed on cells stimulated overnight with either H- restricted specific Envelope JR-9mer peptide or H- restricted unrelated GFP-9mer peptide. IFN- producing T cells are expressed as SFC/106 cells after background subtraction (see Section 2). The error bars indicate the standard deviation among four mice of the same group, and -values are indicated for intergroup comparisons.