Two-Dimensional Liquid Chromatography Technique Coupled with Mass Spectrometry Analysis to Compare the Proteomic Response to Cadmium Stress in Plants

Figure 2

Magnified area of a virtual gel obtained after 2nd dimension separation (HPRC), for the 28th pH fraction, and reproduced by “DeltaVue” software. The protein pattern of untreated plants (0 𝜇M CdSO₄) is shown in shades of blue on the left and the protein pattern of treated plants (50 𝜇M CdSO₄) is shown in shades of yellow on the right. The center column demonstrates the differences in protein abundance between the control and the treated protein samples, as indicated by arrows at the peaks/ bands for proteins of different abundances.