Increased Expression of Ganglioside GM1 in Peripheral CD4+ T Cells Correlates Soluble Form of CD30 in Systemic Lupus Erythematosus Patients

Figure 2

Lipid raft/GM1 expression on CD4+ T cells (a) and CD8+ T cells (b). SLE patients were divided into subgroups; active SLE group (𝑛=21) versus inactive SLE group (𝑛=23) according to SLEDAI scores, and high IgG group (𝑛=24) versus normal IgG group (𝑛=20) according to serum IgG level. ^∗Statistically significant differences compared to the healthy (^∗𝑃<.05,^∗∗𝑃<.01). #Statistically significant differences between subgroup with high IgG and normal IgG. (𝑃<.01) (Two-tailed unpaired Student’s t-test). (c) Percentage of CD4-positive T cells among healthy controls (𝑛=23), inactive (𝑛=11) and active SLE patients (𝑛=13) were 46.9+2.17, 42.2+3.04 and 38.8+2.64, respectively. (d) GM1 expression on CD45RO+/CD4+ memory T cells by triple staining. Cells were first gated by CD4-APC, then analyzed with PE-CD45RO antibody and FITC-GM1. Cells with more than 10 in MFI of GM1 were determined as high GM1 cells. (e) Percent of high GM1-expessing CD45RO+/CD4+ memory T cells among normal controls, inactive and active SLE patients (0.69±0.03, 1.00±0.07, 1.35±0.12, resp.).^∗∗𝑃<.01

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