iTRAQ Quantitative Analysis of Multidrug Resistance Mechanisms in Human Gastric Cancer Cells
Figure 1
(a) Flow chart of iTRAQ proteomics approach. (b) A representative MS/MS spectrum showing the peptides from Sorcin (peptide sequence: FISFDTDR). The cells SGC7901/VCR and SGC7901 were labeled with iTRAQ reagents 121 and 119. So the ratio of 121 : 119 would indicate the relative abundance of Sorcin protein in vincristine-resistant SGC7901/VCR versus SGC7901 (c).
