Development of Th1 Imprints to rBCG Expressing a Foreign Protein: Implications for Vaccination against HIV-1 and Diverse Influenza Strains
Figure 1
Immune response of four individual mice per group after i.v. immunization with different doses of recombinant BCG (rBCGgal) or control rBCG expressing HIV gag protein (rBCGHgag). Immunization dose is indicated below each graph. Mice were immunized as indicated under each graph. After 9 weeks, the mice were killed and the number of -gal- and BCG-specific IFN- and IL-4-producing cells in their spleens was determined by ELISPOT assay. The response in mice given a high dose of BCG transformed with a control vector containing the HIV gag sequence is also shown. Open bars—IFN response to BCG; Black bars—IFN response to -gal; Dark gray bars—IL-4 response to BCG; Light gray bars—IL-4 response to -gal. All values were corrected for background by subtracting the number of spots observed in wells without antigen. Results are representative of two independent experiments.
