Development of Th1 Imprints to rBCG Expressing a Foreign Protein: Implications for Vaccination against HIV-1 and Diverse Influenza Strains
Figure 3
Immune response of individual mice vaccinated with different doses of rBCGβgal after subsequent i.d. challenge with a high dose of rBCGβgal. Each group of mice was initially given an i.d. vaccination dose of rBCGβgal as indicated below each graph with a control group receiving challenge dose only shown. After 4 months, the mice were challenged with approximately 2 107 rBCGβgal cfu given i.d. After an additional 16 weeks, the mice were killed and the number of β-gal- and BCG-specific IFNγ- and IL-4-producing cells in their spleens was determined by ELISPOT assay. Open bars—IFNγ response to BCG; Black bars—IFNγ response to β-gal; Dark gray bars—IL-4 response to BCG; Light gray—IL-4 response to β-gal. All values were corrected for background by subtracting the number of spots observed in wells without antigen. Results are representative of two independent experiments.
