Development of Th1 Imprints to rBCG Expressing a Foreign Protein: Implications for Vaccination against HIV-1 and Diverse Influenza Strains
Figure 6
Number of IFNγ- and IL-4-producing cells in mice immunized i.p. with soluble β-gal (A) and β-gal adsorbed to alum as an adjuvant (B). Soluble β-gal was administered twice five days apart, while β-gal/alum was administered only once. Dose of β-gal administered in each experiment is indicated (10 g or 100 g). Mice were killed 11 days after the initial injection, and antigen-specific cytokine-producing spleen cells were enumerated by ELISPOT assay. Open bars represent IFNγ spots and black bars represent IL-4 spots. Results are representative of three separate experiments.
