Semi-Automated Library Preparation for High-Throughput DNA Sequencing Platforms
Figure 3
Comparison between the purification methods used in PSALM (CarboxyBeads) and traditional manual spin column preparation (Qiagen) is presented in the histogram (a), while histogram (b) plots the average CT values obtained for quantification of barcoded libraries prepared according to PSALM. The error bars represent a 95% confidence interval. The carboxy bead method recovers an average of 93% of the initial DNA (a), while libraries prepared using the semi-automated pipeline are amplified with same number of PCR cycles (Average CT ), which indicates similar concentration of libraries in the tested samples.
