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Journal of Biomedicine and Biotechnology
Volume 2010 (2010), Article ID 620827, 7 pages
http://dx.doi.org/10.1155/2010/620827
Research Article

Oral Microencapsulated Live Saccharomyces cerevisiae Cells for Use in Renal Failure Uremia: Preparation and In Vivo Analysis

Biomedical Technology and Cell Therapy Research Laboratory, Department of Biomedical Engineering, Faculty of Medicine, McGill University, Duff Medical Building, 3775 University Street, Montréal, QC, Canada H3A 2B4

Received 9 February 2010; Accepted 21 April 2010

Academic Editor: Ali Khraibi

Copyright © 2010 Razek Coussa et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Orally administrable alginate-poly-L-lysine-alginate (APA) microcapsules containing live yeast cells was investigated for use in renal failure. At all times, yeast cells remain inside the microcapsules, which are then excreted in the stool. During their gastrointestinal passage, small molecules, like urea, diffuse into the yeast microcapsules where they are hydrolyzed. Orally administrating these microcapsules to uremic rats was found to decrease urea concentrations from  mmol/L to  mmol/L over a treatment period of eight weeks. After stopping the treatment, the urea concentrations increased back to uremic levels of  mmol/L. The analysis of creatinine concentrations averaged mol/L, mol/L, and mol/L for the normal-control, uremic-control and uremic-treatment groups, respectively. While creatinine concentrations for both uremic-control and uremic-treatment groups did not differ among each other ( ), they were, however, significantly higher than those of the normal control group ( ). Uric acid concentrations averaged mol/L, mol/L, and mol/L for the normal-control, uremic-control and uremic-treatment groups, respectively. There were no significant differences in both calcium and phosphate concentrations among all three groups ( ). The microbial populations of five tested types of bacteria were not substantially altered by the presence of the yeast APA encapsulated yeast ( ).