200 g naked RNA intradermally, biweekly for 8 weeks, followed by monthly injections for 6 months. 150 g GM-CSF subcutaneously 24 h after RNA injection.
Arm 1: 3.2–80 g RNA per antigen + 128 g protamine intradermally on days 1, 3, 5, weeks 2, 3, 4, 5, 6, 7, 11, 15, 19. 200 g GM-CSF subcutaneously 24 h after RNA injection. Arm 2: 3.2–80 g RNA per antigen + 128 g protamine + 4 mg KLH intradermally on days 1, 3, 5, weeks 2, 3, 4, 5, 6, 7, 11, 15, 19. 200 g GM-CSF subcutaneously 24 h after RNA injection.
Arm 1: 20 g naked RNA per antigen intradermally on days 0, 14, 28, 42, followed by monthly injections. 100 g/m2 GM-CSF subcutaneously 24 h after RNA injection. Arm 2: 50 g naked RNA per antigen intradermally on days 0–3, 7–10, 28, 42, followed by monthly injections. 250 g/m2 GM-CSF subcutaneously 24 h after RNA injection.