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Journal of Biomedicine and Biotechnology
Volume 2010 (2010), Article ID 645213, 10 pages
http://dx.doi.org/10.1155/2010/645213
Research Article

๐›ฝ -Glucan Oligosaccharide Enhances C D ๐Ÿ– + T Cells Immune Response Induced by a DNA Vaccine Encoding Hepatitis B Virus Core Antigen

1Key laboratory of Medical Molecular Virology of Ministries of Education and Health, Institute of Medical Microbiology and Institutes of Biomedical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032, China
2Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, Nanjing 210038, China
3Department of Biochemistry and Molecular Biology, Shanghai Medical College, Fudan University, Shanghai 200032, China
4State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
5Laboratory of Cytokines, Unit of Immunobiology, Institute of Biomedical Technologies, National Research Council, 56124 Pisa, Italy

Received 30 November 2009; Revised 27 February 2010; Accepted 29 March 2010

Academic Editor: Zhengguo Xiao

Copyright © 2010 Jing Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

DNA vaccination can induce specific C D 8 + T cell immune response, but the response level is low in large mammals and human beings. Coadministration of an adjuvant can optimize protective immunity elicited by a DNA vaccine. In this study, we investigated the effect of a synthetic glucohexaose ( ๐›ฝ -glu6), an analogue of Lentinan basic unit, on specific C D 8 + T cell response induced by a DNA vaccine encoding HBcAg (pB144) in mice. We found that ๐›ฝ -glu6 promoted the recruitment and maturation of dendritic cells, enhanced the activation of C D 8 + and C D 4 + T cells and increased the number of specific C D 8 + /IFN- ๐›พ + T cells in lymphoid and nonlymphoid tissues in mice immunized by pB144. Immunization with pB144 and ๐›ฝ -glu6 increased the anti-HBc IgG and IgG2a antibody titer. These results demonstrate that ๐›ฝ -glu6 can enhance the virus-specific CTL and Th1 responses induced by DNA vaccine, suggesting ๐›ฝ -glu6 as a candidate adjuvant in DNA vaccination.