𝛽-Glucan Oligosaccharide Enhances CD𝟖⁺ T Cells Immune Response Induced by a DNA Vaccine Encoding Hepatitis B Virus Core Antigen

Figure 4

Effect of 𝛽-glu6 on the CD8⁺/IFN-𝛾⁺ T cells induced by pB144 in the spleen. Mice were immunized by pB144 with 𝛽-glu6, and boosted at day 30 after priming. At day 5 (left) and 14 (middle) after priming, and day 10 after boosting (right), splenocytes were isolated and cultured for 5 hours in the absence or in the presence of a specific peptide corresponding to K^b-restricted CTL epitope of HBcAg. Cells were then double-stained with PerCP-anti-CD8a and APC-anti-IFN-𝛾 antibodies and analyzed cytofluorimetrically. Data expressed as mean ± SD of 3 mice/group. *𝑃<.05, pB144+G versus pB144 alone.