Figure 4: Effect of -glu6 on the CD8+/IFN-+T cells induced by pB144 in the spleen. Mice were immunized by pB144 with -glu6, and boosted at day 30 after priming. At day 5 (left) and 14 (middle) after priming, and day 10 after boosting (right), splenocytes were isolated and cultured for 5 hours in the absence or in the presence of a specific peptide corresponding to -restricted CTL epitope of HBcAg. Cells were then double-stained with PerCP-anti-CD8a and APC-anti-IFN- antibodies and analyzed cytofluorimetrically. Data expressed as mean SD of 3 mice/group. *, pB144+G versus pB144 alone.