Figure 5: Effect of -glu6 on the CD8+/IFN-+
T cells induced by pB144 in different organs. Mice were immunized with pB144 and treated with -glu6, and boosted at day 30. Ten days after boosting, the liver lymphocytes, PBMC, and splenocytes were isolated and cultured for 5 hours in the absence or in the presence of a specific peptide corresponding to -restricted CTL epitope of HBcAg. Cells were then double-stained with PE-anti-CD8a and APC-anti-IFN- antibodies and analyzed cytofluorometrically. Data are expressed as mean SD of 3 mice/group. *, pB144+G versus pB144 alone.