|Table 1: Effect of -glu6 on pB144-induced antibody production*.|
*The sera from immunized mice were collected 4 weeks after priming and 2 weeks after boosting. Anti-HBcAg IgG, IgG1, and IgG2a were analyzed by ELISA. The initial dilution of each serum from immunized mouse was 1 : 100 and followed with serial of three-fold dilution for anti-HBc IgG detection, and two-fold dilution for anti-HBc IgG1, IgG2a analysis. |
**The well with an absorbance O 0.20 (the blank well, ~0.020) was scored as positive. The anti-HBc antibody titers are expressed as the reciprocal of the highest dilution showing a positive reaction and calculated as the mean ± standard deviation (SD) of for each group (6 mice/group).
***N.D. indicates that anti-HBc antibody in the sera of the mice is under detection level.
**** , pB144 +G versus pB144 alone.