Table 1: Effect of 𝛽 -glu6 on pB144-induced antibody production*.

ImmunogensAnti-HBcAg antibody titer ( L o g 2 ± SD)**
Week 4 after primingWeek 2 after boosting
IgGIgG1IgG2aIgGIgG1IgG2a

GN.D. *** N.D.N.D.N.D. N.D.N.D.
pB144 9 . 8 1 ± 0 . 2 1 N.D.N.D. 1 2 . 9 8 ± 0 . 1 8 N.D. 1 1 . 8 1 ± 0 . 5 5
pB144+G 1 1 . 4 0 ± 0 . 0 9 **** N.D. 6 . 9 0 ± 0 . 4 9 1 4 . 5 7 ± 0 . 5 0 **** N.D. 1 2 . 1 3 ± 0 . 6
PBSN.D.N.D.N.D.N.D.N.D.N.D.

*The sera from immunized mice were collected 4 weeks after priming and 2 weeks after boosting. Anti-HBcAg IgG, IgG1, and IgG2a were analyzed by ELISA. The initial dilution of each serum from immunized mouse was 1 : 100 and followed with serial of three-fold dilution for anti-HBc IgG detection, and two-fold dilution for anti-HBc IgG1, IgG2a analysis.
**The well with an absorbance O D 4 5 0  0.20 (the blank well, ~0.020) was scored as positive. The anti-HBc antibody titers are expressed as the reciprocal of the highest dilution showing a positive reaction and calculated as the mean ± standard deviation (SD) of L o g 2 for each group (6 mice/group).
***N.D. indicates that anti-HBc antibody in the sera of the mice is under detection level.
**** 𝑃 < . 0 5 , pB144 +G versus pB144 alone.