Table 1: List of DIGE-identified proteins with a changed abundance in 9-month old dystrophic MDX heart muscle.

Spot No.Protein nameProtein IDPeptides matchedCoverage (%)Molecular mass (kDa)Isoelectric point (pI)Mascot scoreFold change

1Nucleoside diphosphate kinase BNDKB_MOUSE43417.56.9694.4
2Lamin-A/CLMNA_MOUSE4674.56.51111.9
3Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrialETFD_MOUSE81568.97.3451.8
4Myozenin-2MYOZ2_MOUSE42029.88.51450.67
5Electron transfer flavoprotein subunit alpha, mitochondrialETFA_MOUSE21235.38.6890.67
6ProhibitinPHB_MOUSE84129.95.62900.67
7Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrialODO2_MOUSE3649.39.1860.63
8Isovaleryl-CoA dehydrogenase, mitochondrialIVD_MOUSE72346.78.5890.59
9Serum albuminALBU_MOUSE3570.75.8820.59
10Cytochrome b-c1 complex subunit Rieske, mitochondrialUCRI_MOUSE41329.68.9840.56
11Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrialODP2_MOUSE102068.58.81170.53
12VimentinVIME_MOUSE112053.75.11180.53
13Beta-enolaseENOB_MOUSE81947.36.71110.50
14Pyruvate dehydrogenase E1 component subunit beta, mitochondrialODPB_MOUSE83139.36.42790.50
15Pyruvate dehydrogenase E1 component subunit beta, mitochondrialODPB_MOUSE31939.36.41340.50
16Creatine kinase M-typeKCRM_MOUSE2443.36.6470.50
17Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrialECH1_MOUSE42436.47.62010.50
18Heat shock protein beta-1 Hsp27HSPB1_MOUSE2823.16.1500.50
19Isovaleryl-CoA dehydrogenase, mitochondrialIVD_MOUSE71746.78.5980.50
20Pyruvate dehydrogenase protein X component, mitochondrialODPX_MOUSE51154.37.6630.48
21Actin, alpha cardiac muscle 1ACTC_MOUSE61942.35.21040.48
22Cytochrome c oxidase subunit 5B, mitochondrialCOX5A_MOUSE21414.18.7520.48
23VimentinVIME_MOUSE132753.75.11220.48
24Transitional endoplasmic reticulum ATPase (valosin-containing protein VCP)TERA_MOUSE51089.95.11710.46
25Pyruvate dehydrogenase E1 component subunit beta, mitochondrialODPB_MOUSE41439.36.41000.44
26Myosin regulatory light chain 2, ventricular/cardiac muscle isoformMLRV_MOUSE95218.94.92090.44
27Fatty acid-binding protein FABP-3, heartFABPH_MOUSE53714.86.11490.44
28Enolase 1, alphaENOA_MOUSE2347.56.4430.44
29DJ-1 proteinPARK7_MOUSE42620.26.3900.44
30Cytochrome b-c1 complexQCR1_MOUSE61753.45.8830.44
3160 kDa heat shock protein, Hsp60, mitochondrialCH60_MOUSE2661.15.9630.42
32Beta-enolaseENOB_MOUSE3747.36.7920.42
33Fatty acid binding protein, FABP-3, heartFABPH_MOUSE75214.86.11620.42
34Pyruvate dehydrogenase protein X component, mitochondrialODPX_MOUSE2454.37.6660.42
35Tropomyosin alpha-1 chainTPM1_MOUSE154332.74.73020.40
36Electron transfer flavoprotein subunit alpha, mitochondrialETFA_MOUSE83335.38.62350.40
3760 kDa heat shock protein, Hsp60, mitochondrialCH60_MOUSE123261.15.93460.40
38Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrialIDH3A_MOUSE92940.16.31640.40
39Actin, alpha cardiac muscle 1ACTC_MOUSE123142.35.23170.39
40NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrialNDUV2_MOUSE62927.67.03700.39
41Voltage-dependent anion-selective channel protein VDAC1VDAC1_MOUSE102932.58.62920.39
42Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrialIDH3A_MOUSE133940.16.33760.39
43DesminDESM_MOUSE305053.55.211840.39
4460 kDa heat shock protein, Hsp60, mitochondrialCH60_MOUSE253861.15.98420.37
45Actin, alpha cardiac muscle 1ACTC_MOUSE102642.35.22760.36
46Actin, alpha cardiac muscle 1ACTC_MOUSE41442.35.2880.36
47DesminDESM_MOUSE153753.55.23490.36
48Myosin regulatory light chain 2, atrial isoformMLRA_MOUSE95719.64.82990.36
49Fatty acid-binding protein, FABP-3, heartFABPH_MOUSE126114.86.14250.35
50ATP synthase subunit beta, mitochondrialATPB_MOUSE184056.35.210930.35
51Cytochrome c oxidase subunit 5ACOX5A_MOUSE72816.36.12860.35
52DesminDESM_MOUSE266253.55.216570.35
53Myosin light chain 3MYL3_MOUSE64122.55.0900.35
54DesminDESM_MOUSE123053.55.24310.33
55ATP synthase subunit d, mitochondrialATP5H_MOUSE32118.85.5480.33
56Actin, alpha cardiac muscle 1ACTC_MOUSE164042.35.25980.32
57Cytochrome b-c1 complexQCR1_MOUSE122753.45.83300.32
58DesminDESM_MOUSE366853.55.212890.32
59Tropomyosin alpha-1 chainTPM1_MOUSE214632.74.76050.32
60Actin, alpha cardiac muscle 1ACTC_MOUSE185942.35.24500.31
61Myosin light chain 3MYL3_MOUSE116322.55.02310.30
62Myosin regulatory light chain 2, ventricular/cardiac muscle isoformMLRV_MOUSE137318.94.91180.29
63Cytochrome b-c1 complexQCR1_MOUSE123153.45.83420.29
64Actin, alpha cardiac muscle 1ACTC_MOUSE73642.35.21600.29
65Myosin regulatory light chain 2, ventricular/cardiac muscle isoformMLRV_MOUSE74618.94.93540.29
66Actin, alpha cardiac muscle 1ACTC_MOUSE42442.35.2910.28
67Actin, alpha cardiac muscle 1ACTC_MOUSE176242.35.22940.27
68Adenylate kinase isoenzyme 1KAD1_MOUSE53121.65.71740.26
69ATP synthase subunit beta, mitochondrialATPB_MOUSE42156.35.2920.26
70Myosin regulatory light chain 2MLRA_MOUSE64719.64.81680.25
71Actin, alpha cardiac muscle 1ACTC_MOUSE186142.35.23020.25
72ATP synthase subunit d, mitochondrialATP5H_MOUSE53618.85.5690.25
73ATP synthase subunit beta, mitochondrialATPB_MOUSE185156.35.26400.25
74ATP synthase subunit beta, mitochondrialATPB_MOUSE257156.35.211020.25
75Actin, alpha cardiac muscle 1ACTC_MOUSE61642.35.21190.25
76Myosin light chain 3MYL3_MOUSE63022.55.0990.25
77ATP synthase subunit beta, mitochondrialATPB_MOUSE247056.35.28710.24
78Myosin light chain 2, ventricular/cardiacMLRV_MOUSE117718.94.92060.24
79Peroxiredoxin-6PRDX6_MOUSE31924.95.71020.19