Microtubules Growth Rate Alteration in Human Endothelial Cells

Figure 3

Microtubule plus-ends growth rates are different in centrosome region and on the periphery near the cell margin. Growing microtubule plus-ends were selectively marked in human EC. HPAEC were transfected with the plasmid expressing EB3-GFP. Persistent microtubule growth was confirmed by long EB3-GFP tracks. EB3-GFP movement was analyzed by time-lapse microscopy. Images were acquired every 1 second. Histogram of microtubule growth rate distribution were obtained by tracking EB3-GFP comets at microtubule plus-ends in HPAEC in the centrosome region (mean growth rate, 16.7±0.3 𝜇m/min (𝑛=82)) (a) and near the cell margin (mean growth rate, 12.9±0.1 𝜇m/min (𝑛=300)) (b).

(a)

(b)