Telomere Attrition Occurs during Ex Vivo Expansion of Human Dental Pulp Stem Cells
Figure 4
Immunocytochemical detection of analyzed markers in cultured DPSCs. (a) STRO-1 expression was readily detectable in all cells. (b) Immunostaining of vimentin revealed a regular filamentous cytoskeletal network within the cells with signal accentuation in perinuclear region. (c) Nestin expression appeared in the cell body, however, in cytoplasmic outgrowths the signal was lower but still distinguishable. Intensive signal is localized in dividing cells. (d) CXCR4 was localized mainly in the cytoplasm, namely, in its perinuclear area; some signal was also distinguishable within the cell nuclei. (e) Sox2 was distributed mainly in cell nuclei; signal in perinuclear cytoplasm likely reflects synthesis of this transcription factor in endoplasmic reticulum. (f) Nucleostemin was observed in nodular structures in cell nuclei; in mitotic cells or cells that had just finished cytokinesis (arrowheads) immunoreactivity was lost from the nucleus and distributed diffusely. (g) CD90 immunofluorescence showed homogenous dot-like membrane staining pattern. (h) CD166 was localised in membrane of all DPSCs contouring the cell edges. Nuclei were counterstained with DAPI. Arrows indicate dividing cells. Scale bars 50 m.
